Coding

Part:BBa_K1680013:Design

Designed by: Katharina Sporbeck   Group: iGEM15_Tuebingen   (2015-09-17)

NDegron

Contains a NLS (Nuclear localisation sequence) followed by a spacer and a modified TEV-cleavage site. After Protease cleavage Phenylalanine is exposed N-terminally, which leads to rapid protein depletion. In addition to that, after cleavage a NES (Nuclear export sequence) is exposed.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Shipped in RFC25


Source

gBlock designed by Team Tuebingen

References

Taxis, Christof, et al. "Efficient protein depletion by genetically controlled deprotection of a dormant N‐degron." Molecular systems biology 5.1 (2009): 267.